Skill: image analysis
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Tracking of cancer cell-matrix interactions in a fully controllable 3D fiber system
The mechanical, geometrical and chemical properties of the 3D fibrous extracellular matrix play a key role in cancer initiation and progression, by controlling the movement and fate of tumor cells as well as surrounding fibroblasts, immune or endothelial cells. A strong emphasis has been put recently on the development of matrices with controllable fiber properties…
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High-throughput single-molecule FISH to calibrate in-vivo transcriptional kinetics of developmental timing genes in C. elegans
Spatiotemporal patterns of gene expression are fundamental to every developmental program. The resulting gene expression domains are traditionally characterized by their levels of gene products. However, in vivo transcriptional dynamics are known to be highly dynamic, often stochastically fluctuating between periods of quiescence and periods of pronounced transcriptionally activity. To fully understand how patterns of…