Skill: optics
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Simultaneous 3D localization and 2D orientation determination for single-molecules super resolution microscopy
Super-resolution (SR) microscopy have revolutionized our understanding of the biological processes at the molecular level. Imaging single molecules and localizing their center with high precision allows the reconstruction of pointillist image of the sample with a resolution beyond the diffraction limit [1,2,3]. In order to capture the 3D nanoscale morphology of the whole cell, multifocus…
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Multicolor multifocus microscopy for multidimensional single molecule imaging
The ability to control the wavefront at the output of an imaging system allows to correct the imaging system optical aberration but also to impose a controlled deformation to encode specific information in the recorded images. Diffractive optical elements (DOE) provide an efficient mean to manipulate light. When placed in the back focal plane of…