Skill: imaging
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Mechanisms of renal cyst formation studied with biomimetic kidney-on-chips
Genetic renal kidney diseases lead to the development of numerous cysts in the renal tubules, ultimately leading to kidney failure. Despite extensive work on the genetics and cell biology of these diseases, the precise mechanism of cyst formation, resulting from localized dilatation of the renal tubules, remains imperfectly understood. Renal tubules have an extremely controlled…
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Deciphering protein-induced invagination at sub-nanometric resolution by cryo-electron tomography
Cryo-electron microcopy is revolutionising our vision of proteins and the cell (Nobel Prize 2017). One approach, known as isolated particle analysis, makes it possible to build atomic models of purified and homogeneous. At the same time, cryo-tomography (cryo-ET) is being developed to obtain molecular models of more complex samples, in vitro reconstituted or within cells…