Keyword: C. elegans
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High-throughput single-molecule FISH to calibrate in-vivo transcriptional kinetics of developmental timing genes in C. elegans
Spatiotemporal patterns of gene expression are fundamental to every developmental program. The resulting gene expression domains are traditionally characterized by their levels of gene products. However, in vivo transcriptional dynamics are known to be highly dynamic, often stochastically fluctuating between periods of quiescence and periods of pronounced transcriptionally activity. To fully understand how patterns of…